The latest report from the national infrastructure body shows Queensland is suffering the largest drop in investor confidence in Australia.
LNP Deputy Leader Tim Mander said investor preference in Queensland infrastructure had plummeted because of the Palaszczuk Labor Government.
"Queensland has had the single biggest drop in preference out of any state or territory in Australia," Mr Mander said.
"The ideologically-driven Labor Government is stopping investors coming to Queensland.
"New South Wales and Victoria are now nearly three times more attractive to those wanting to invest in infrastructure than Queensland.
"Queensland is struggling with the nation's highest unemployment rate, record congestion and skyrocketing cost of living.
"Labor's anti-business agenda is hurting Queenslanders because we are losing out on billions of dollars in desperately needed infrastructure investment.
"Less than 10 per cent of investors thought that the Palaszczuk Labor Government was easy to work with.
"Both New South Wales and Victoria were ranked more than five times higher for ease of working with."
Mr Mander said no state asset would be sold or leased under an LNP Government.
"The findings in this report are a result of Labor's addiction to taxes," Mr Mander said.
"You can't tax your way to growth, it's as simple as that.
"Queensland needs a government that answers to Queenslanders, not union bosses."
The Australian Infrastructure Investment Report for 2018 showed 21 per cent of participants named Queensland as a preferred investment destination, compared to 35 per cent in 2017.